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Addgene

pCasper4-g-osk-TRICK
(Plasmid #71244)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71244 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCasper4
  • Vector type
    Insect Expression ; P element-mediated germline transformation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    oskar
  • Alt name
    osk
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    osk (a.k.a. Dmel_CG10901, CG10901, Dm-osk, Dmel\CG10901, OSK, Osk, Oskar, norka)
  • Promoter osk
  • Tags / Fusion Proteins
    • 6xMS2
    • 12xPP7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCasper-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCasper4-g-osk-TRICK was a gift from Anne Ephrussi (Addgene plasmid # 71244 ; http://n2t.net/addgene:71244 ; RRID:Addgene_71244)
  • For your References section:

    Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals. Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer RH, Chao JA. Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380. 10.1126/science.aaa3380 PubMed 25792328