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PurposeUsed to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71242 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHsp83
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Vector typeInsect Expression ; P element-mediated germline transformation
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNLS-2xMCP-2xTagRFP-T
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Alt nameNLS-MCP-RFP
- Promoter Hsp83
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Tag
/ Fusion Protein
- TagRFP-T (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pCasper-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHsp83-NLS-HA-2xMCP-2xTagRFP-T was a gift from Anne Ephrussi (Addgene plasmid # 71242 ; http://n2t.net/addgene:71242 ; RRID:Addgene_71242) -
For your References section:
Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals. Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer RH, Chao JA. Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380. 10.1126/science.aaa3380 PubMed 25792328
Map uploaded by the depositor.
