pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro Notes
Addgene Diagnostic Digest- MluI
This gel depicts the NEB 1kb extend ladder and a digestion of the plasmid pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro with the following enzyme: MluI-HF.
Addgene Diagnostic Digest- NotI/KpnI
This gel depicts the NEB 1kb ladder and a digestion of the plasmid pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro with the following enzymes: NotI-HF and KpnI-HF.
We observe the expected two bands: ~8.8kb and 6.1kb
