pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro Citations (93)
Originally described in: Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements.Thakore PI, D'Ippolito AM, Song L, Safi A, Shivakumar NK, Kabadi AM, Reddy TE, Crawford GE, Gersbach CA Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26. PubMed Journal
Articles Citing pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
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| Dissecting super-enhancer hierarchy based on chromatin interactions. Huang J, Li K, Cai W, Liu X, Zhang Y, Orkin SH, Xu J, Yuan GC. Nat Commun. 2018 Mar 5;9(1):943. doi: 10.1038/s41467-018-03279-9. PubMed |
| High-throughput screening of prostate cancer risk loci by single nucleotide polymorphisms sequencing. Zhang P, Xia JH, Zhu J, Gao P, Tian YJ, Du M, Guo YC, Suleman S, Zhang Q, Kohli M, Tillmans LS, Thibodeau SN, French AJ, Cerhan JR, Wang LD, Wei GH, Wang L. Nat Commun. 2018 May 22;9(1):2022. doi: 10.1038/s41467-018-04451-x. PubMed |
| A Muscle-Specific Enhancer RNA Mediates Cohesin Recruitment and Regulates Transcription In trans. Tsai PF, Dell'Orso S, Rodriguez J, Vivanco KO, Ko KD, Jiang K, Juan AH, Sarshad AA, Vian L, Tran M, Wangsa D, Wang AH, Perovanovic J, Anastasakis D, Ralston E, Ried T, Sun HW, Hafner M, Larson DR, Sartorelli V. Mol Cell. 2018 Jul 5;71(1):129-141.e8. doi: 10.1016/j.molcel.2018.06.008. PubMed |
| Activating PTEN Tumor Suppressor Expression with the CRISPR/dCas9 System. Moses C, Nugent F, Waryah CB, Garcia-Bloj B, Harvey AR, Blancafort P. Mol Ther Nucleic Acids. 2019 Mar 1;14:287-300. doi: 10.1016/j.omtn.2018.12.003. Epub 2018 Dec 14. PubMed |
| The Crohn's disease associated SNP rs6651252 impacts MYC gene expression in human colonic epithelial cells. Matthews SM, Eshelman MA, Berg AS, Koltun WA, Yochum GS. PLoS One. 2019 Feb 22;14(2):e0212850. doi: 10.1371/journal.pone.0212850. eCollection 2019. PubMed |
| Increased Serine Synthesis Provides an Advantage for Tumors Arising in Tissues Where Serine Levels Are Limiting. Sullivan MR, Mattaini KR, Dennstedt EA, Nguyen AA, Sivanand S, Reilly MF, Meeth K, Muir A, Darnell AM, Bosenberg MW, Lewis CA, Vander Heiden MG. Cell Metab. 2019 Jun 4;29(6):1410-1421.e4. doi: 10.1016/j.cmet.2019.02.015. Epub 2019 Mar 21. PubMed |
| Hominoid-Specific Transposable Elements and KZFPs Facilitate Human Embryonic Genome Activation and Control Transcription in Naive Human ESCs. Pontis J, Planet E, Offner S, Turelli P, Duc J, Coudray A, Theunissen TW, Jaenisch R, Trono D. Cell Stem Cell. 2019 May 2;24(5):724-735.e5. doi: 10.1016/j.stem.2019.03.012. Epub 2019 Apr 18. PubMed |
| The enhancer RNA ARIEL activates the oncogenic transcriptional program in T-cell acute lymphoblastic leukemia. Tan SH, Leong WZ, Ngoc PCT, Tan TK, Bertulfo FC, Lim MC, An O, Li Z, Yeoh AEJ, Fullwood MJ, Tenen DG, Sanda T. Blood. 2019 Jul 18;134(3):239-251. doi: 10.1182/blood.2018874503. Epub 2019 May 10. PubMed |
| Inhibition of nucleotide synthesis promotes replicative senescence of human mammary epithelial cells. Delfarah A, Parrish S, Junge JA, Yang J, Seo F, Li S, Mac J, Wang P, Fraser SE, Graham NA. J Biol Chem. 2019 Jul 5;294(27):10564-10578. doi: 10.1074/jbc.RA118.005806. Epub 2019 May 28. PubMed |
| An Oxygen-Dependent Interaction between FBXL5 and the CIA-Targeting Complex Regulates Iron Homeostasis. Mayank AK, Pandey V, Vashisht AA, Barshop WD, Rayatpisheh S, Sharma T, Haque T, Powers DN, Wohlschlegel JA. Mol Cell. 2019 Jul 25;75(2):382-393.e5. doi: 10.1016/j.molcel.2019.05.020. Epub 2019 Jun 19. PubMed |
| CD44 3'-Untranslated Region Functions as a Competing Endogenous RNA to Enhance NK Sensitivity of Liver Cancer Stem Cell by Regulating ULBP2 Expression. Weng J, Han X, Liu K, Yang J, Wei S, Zhang Y, Zeng F, Li Y, Shen L, Gao Y. Int J Biol Sci. 2019 Jun 4;15(8):1664-1675. doi: 10.7150/ijbs.35216. eCollection 2019. PubMed |
| Blockade of MDM2 with inactive Cas9 prevents epithelial to mesenchymal transition in retinal pigment epithelial cells. Liu B, Song J, Han H, Hu Z, Chen N, Cui J, Matsubara JA, Zhong J, Lei H. Lab Invest. 2019 Dec;99(12):1874-1886. doi: 10.1038/s41374-019-0307-9. Epub 2019 Aug 22. PubMed |
| Circular ecDNA promotes accessible chromatin and high oncogene expression. Wu S, Turner KM, Nguyen N, Raviram R, Erb M, Santini J, Luebeck J, Rajkumar U, Diao Y, Li B, Zhang W, Jameson N, Corces MR, Granja JM, Chen X, Coruh C, Abnousi A, Houston J, Ye Z, Hu R, Yu M, Kim H, Law JA, Verhaak RGW, Hu M, Furnari FB, Chang HY, Ren B, Bafna V, Mischel PS. Nature. 2019 Nov;575(7784):699-703. doi: 10.1038/s41586-019-1763-5. Epub 2019 Nov 20. PubMed |
| Functional Enhancers Shape Extrachromosomal Oncogene Amplifications. Morton AR, Dogan-Artun N, Faber ZJ, MacLeod G, Bartels CF, Piazza MS, Allan KC, Mack SC, Wang X, Gimple RC, Wu Q, Rubin BP, Shetty S, Angers S, Dirks PB, Sallari RC, Lupien M, Rich JN, Scacheri PC. Cell. 2019 Nov 27;179(6):1330-1341.e13. doi: 10.1016/j.cell.2019.10.039. Epub 2019 Nov 21. PubMed |
| ASCL1 is a MYCN- and LMO1-dependent member of the adrenergic neuroblastoma core regulatory circuitry. Wang L, Tan TK, Durbin AD, Zimmerman MW, Abraham BJ, Tan SH, Ngoc PCT, Weichert-Leahey N, Akahane K, Lawton LN, Rokita JL, Maris JM, Young RA, Look AT, Sanda T. Nat Commun. 2019 Dec 9;10(1):5622. doi: 10.1038/s41467-019-13515-5. PubMed |
| NRP1 promotes cell migration and invasion and serves as a therapeutic target in nasopharyngeal carcinoma. Huang Z, Cheng C, Xiong H, Wang Y, Chen KK, Yang J, Xiao B, Zhang R, Li S, Sang Y. Int J Clin Exp Pathol. 2018 May 1;11(5):2460-2469. eCollection 2018. PubMed |
Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing.
Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, Yu A, Kaphle P, Dickerson KE, Ni M, Xu J.
Nat Commun. 2020 Jan 24;11(1):485. doi: 10.1038/s41467-020-14362-5.
PubMed
Associated Plasmids |
| Targeting Oncogenic Super Enhancers in MYC-Dependent AML Using a Small Molecule Activator of NR4A Nuclear Receptors. Call SG, Duren RP, Panigrahi AK, Nguyen L, Freire PR, Grimm SL, Coarfa C, Conneely OM. Sci Rep. 2020 Feb 18;10(1):2851. doi: 10.1038/s41598-020-59469-3. PubMed |
| Noncoding Variants Connect Enhancer Dysregulation with Nuclear Receptor Signaling in Hematopoietic Malignancies. Li K, Zhang Y, Liu X, Liu Y, Gu Z, Cao H, Dickerson KE, Chen M, Chen W, Shao Z, Ni M, Xu J. Cancer Discov. 2020 May;10(5):724-745. doi: 10.1158/2159-8290.CD-19-1128. Epub 2020 Mar 18. PubMed |
| CRISPRi-mediated functional analysis of lung disease-associated loci at non-coding regions. Stuart WD, Guo M, Fink-Baldauf IM, Coleman AM, Clancy JP, Mall MA, Lim FY, Brewington JJ, Maeda Y. NAR Genom Bioinform. 2020 Jun;2(2):lqaa036. doi: 10.1093/nargab/lqaa036. Epub 2020 May 25. PubMed |
| Transcriptional repression of PTEN in neural cells using CRISPR/dCas9 epigenetic editing. Moses C, Hodgetts SI, Nugent F, Ben-Ary G, Park KK, Blancafort P, Harvey AR. Sci Rep. 2020 Jul 9;10(1):11393. doi: 10.1038/s41598-020-68257-y. PubMed |
| Silencing integrated SIV proviral DNA with TAR-specific CRISPR tools. Smith LM, Hodara VL, Parodi LM, Callery JE, Giavedoni LD. J Med Primatol. 2020 Oct;49(5):269-279. doi: 10.1111/jmp.12494. Epub 2020 Sep 9. PubMed |
| Cholesterol Pathway Inhibition Induces TGF-beta Signaling to Promote Basal Differentiation in Pancreatic Cancer. Gabitova-Cornell L, Surumbayeva A, Peri S, Franco-Barraza J, Restifo D, Weitz N, Ogier C, Goldman AR, Hartman TR, Francescone R, Tan Y, Nicolas E, Shah N, Handorf EA, Cai KQ, O'Reilly AM, Sloma I, Chiaverelli R, Moffitt RA, Khazak V, Fang CY, Golemis EA, Cukierman E, Astsaturov I. Cancer Cell. 2020 Oct 12;38(4):567-583.e11. doi: 10.1016/j.ccell.2020.08.015. Epub 2020 Sep 24. PubMed |
| NFIA differentially controls adipogenic and myogenic gene program through distinct pathways to ensure brown and beige adipocyte differentiation. Hiraike Y, Waki H, Miyake K, Wada T, Oguchi M, Saito K, Tsutsumi S, Aburatani H, Yamauchi T, Kadowaki T. PLoS Genet. 2020 Sep 29;16(9):e1009044. doi: 10.1371/journal.pgen.1009044. eCollection 2020 Sep. PubMed |
| EWS-FLI1 regulates and cooperates with core regulatory circuitry in Ewing sarcoma. Shi X, Zheng Y, Jiang L, Zhou B, Yang W, Li L, Ding L, Huang M, Gery S, Lin DC, Koeffler HP. Nucleic Acids Res. 2020 Nov 18;48(20):11434-11451. doi: 10.1093/nar/gkaa901. PubMed |
| Netrin G1 Promotes Pancreatic Tumorigenesis through Cancer-Associated Fibroblast-Driven Nutritional Support and Immunosuppression. Francescone R, Barbosa Vendramini-Costa D, Franco-Barraza J, Wagner J, Muir A, Lau AN, Gabitova L, Pazina T, Gupta S, Luong T, Rollins D, Malik R, Thapa RJ, Restifo D, Zhou Y, Cai KQ, Hensley HH, Tan Y, Kruger WD, Devarajan K, Balachandran S, Klein-Szanto AJ, Wang H, El-Deiry WS, Vander Heiden MG, Peri S, Campbell KS, Astsaturov I, Cukierman E. Cancer Discov. 2021 Feb;11(2):446-479. doi: 10.1158/2159-8290.CD-20-0775. Epub 2020 Oct 30. PubMed |
| BAHCC1 binds H3K27me3 via a conserved BAH module to mediate gene silencing and oncogenesis. Fan H, Lu J, Guo Y, Li D, Zhang ZM, Tsai YH, Pi WC, Ahn JH, Gong W, Xiang Y, Allison DF, Geng H, He S, Diao Y, Chen WY, Strahl BD, Cai L, Song J, Wang GG. Nat Genet. 2020 Dec;52(12):1384-1396. doi: 10.1038/s41588-020-00729-3. Epub 2020 Nov 2. PubMed |
| Mutations in Spliceosomal Genes PPIL1 and PRP17 Cause Neurodegenerative Pontocerebellar Hypoplasia with Microcephaly. Chai G, Webb A, Li C, Antaki D, Lee S, Breuss MW, Lang N, Stanley V, Anzenberg P, Yang X, Marshall T, Gaffney P, Wierenga KJ, Chung BH, Tsang MH, Pais LS, Lovgren AK, VanNoy GE, Rehm HL, Mirzaa G, Leon E, Diaz J, Neumann A, Kalverda AP, Manfield IW, Parry DA, Logan CV, Johnson CA, Bonthron DT, Valleley EMA, Issa MY, Abdel-Ghafar SF, Abdel-Hamid MS, Jennings P, Zaki MS, Sheridan E, Gleeson JG. Neuron. 2021 Jan 20;109(2):241-256.e9. doi: 10.1016/j.neuron.2020.10.035. Epub 2020 Nov 20. PubMed |
| Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway. Findakly S, Daggubati V, Garcia G, LaStella SA, Choudhury A, Tran C, Li A, Tong P, Garcia JQ, Puri N, Reiter JF, Xu L, Raleigh DR. J Cell Biol. 2021 Jan 4;220(1). pii: 211576. doi: 10.1083/jcb.202002026. PubMed |
| Interaction between SNAI2 and MYOD enhances oncogenesis and suppresses differentiation in Fusion Negative Rhabdomyosarcoma. Pomella S, Sreenivas P, Gryder BE, Wang L, Milewski D, Cassandri M, Baxi K, Hensch NR, Carcarino E, Song Y, Chou HC, Yohe ME, Stanton BZ, Amadio B, Caruana I, De Stefanis C, De Vito R, Locatelli F, Chen Y, Chen EY, Houghton P, Khan J, Rota R, Ignatius MS. Nat Commun. 2021 Jan 8;12(1):192. doi: 10.1038/s41467-020-20386-8. PubMed |
Targeted brachyury degradation disrupts a highly specific autoregulatory program controlling chordoma cell identity.
Sheppard HE, Dall'Agnese A, Park WD, Shamim MH, Dubrulle J, Johnson HL, Stossi F, Cogswell P, Sommer J, Levy J, Sharifnia T, Wawer MJ, Nabet B, Gray NS, Clemons PA, Schreiber SL, Workman P, Young RA, Lin CY.
Cell Rep Med. 2021 Jan 19;2(1):100188. doi: 10.1016/j.xcrm.2020.100188. eCollection 2021 Jan 19.
PubMed
Associated Plasmids |
| Myeloma-specific superenhancers affect genes of biological and clinical relevance in myeloma. Jia Y, Zhou J, Tan TK, Chung TH, Wong RWJ, Chooi JY, Lim JSL, Sanda T, Ooi M, De Mel S, Soekojo C, Chen Y, Zhang E, Cai Z, Shen P, Ruan J, Chng WJ. Blood Cancer J. 2021 Feb 12;11(2):32. doi: 10.1038/s41408-021-00421-7. PubMed |
| Identification of a long non-coding RNA regulator of liver carcinoma cell survival. Rybakova Y, Gonzalez JT, Bogorad R, Chauhan VP, Dong YL, Whittaker CA, Zatsepin T, Koteliansky V, Anderson DG. Cell Death Dis. 2021 Feb 15;12(2):178. doi: 10.1038/s41419-021-03453-w. PubMed |
| FOXF1 is required for the oncogenic properties of PAX3-FOXO1 in rhabdomyosarcoma. Milewski D, Shukla S, Gryder BE, Pradhan A, Donovan J, Sudha P, Vallabh S, Pyros A, Xu Y, Barski A, Szabo S, Turpin B, Pressey JG, Millay DP, Khan J, Kalinichenko VV, Kalin TV. Oncogene. 2021 Mar;40(12):2182-2199. doi: 10.1038/s41388-021-01694-9. Epub 2021 Feb 24. PubMed |
| Creb5 establishes the competence for Prg4 expression in articular cartilage. Zhang CH, Gao Y, Jadhav U, Hung HH, Holton KM, Grodzinsky AJ, Shivdasani RA, Lassar AB. Commun Biol. 2021 Mar 12;4(1):332. doi: 10.1038/s42003-021-01857-0. PubMed |
| Targeting netrin-3 in small cell lung cancer and neuroblastoma. Jiang S, Richaud M, Vieugue P, Rama N, Delcros JG, Siouda M, Sanada M, Redavid AR, Ducarouge B, Hervieu M, Breusa S, Manceau A, Gattolliat CH, Gadot N, Combaret V, Neves D, Ortiz-Cuaran S, Saintigny P, Meurette O, Walter T, Janoueix-Lerosey I, Hofman P, Mulligan P, Goldshneider D, Mehlen P, Gibert B. EMBO Mol Med. 2021 Apr 9;13(4):e12878. doi: 10.15252/emmm.202012878. Epub 2021 Mar 15. PubMed |
| CRISPRi screens reveal a DNA methylation-mediated 3D genome dependent causal mechanism in prostate cancer. Ahmed M, Soares F, Xia JH, Yang Y, Li J, Guo H, Su P, Tian Y, Lee HJ, Wang M, Akhtar N, Houlahan KE, Bosch A, Zhou S, Mazrooei P, Hua JT, Chen S, Petricca J, Zeng Y, Davies A, Fraser M, Quigley DA, Feng FY, Boutros PC, Lupien M, Zoubeidi A, Wang L, Walsh MJ, Wang T, Ren S, Wei GH, He HH. Nat Commun. 2021 Mar 19;12(1):1781. doi: 10.1038/s41467-021-21867-0. PubMed |
| Interpreting type 1 diabetes risk with genetics and single-cell epigenomics. Chiou J, Geusz RJ, Okino ML, Han JY, Miller M, Melton R, Beebe E, Benaglio P, Huang S, Korgaonkar K, Heller S, Kleger A, Preissl S, Gorkin DU, Sander M, Gaulton KJ. Nature. 2021 Jun;594(7863):398-402. doi: 10.1038/s41586-021-03552-w. Epub 2021 May 19. PubMed |
| ASCL1 represses a SOX9(+) neural crest stem-like state in small cell lung cancer. Olsen RR, Ireland AS, Kastner DW, Groves SM, Spainhower KB, Pozo K, Kelenis DP, Whitney CP, Guthrie MR, Wait SJ, Soltero D, Witt BL, Quaranta V, Johnson JE, Oliver TG. Genes Dev. 2021 Jun;35(11-12):847-869. doi: 10.1101/gad.348295.121. Epub 2021 May 20. PubMed |
| Epigenome editing of the CFTR-locus for treatment of cystic fibrosis. Kabadi AM, Machlin L, Dalal N, Lee RE, McDowell I, Shah NN, Drowley L, Randell SH, Reddy TE. J Cyst Fibros. 2021 May 25. pii: S1569-1993(21)00118-1. doi: 10.1016/j.jcf.2021.04.008. PubMed |
| Interplay and cooperation between SREBF1 and master transcription factors regulate lipid metabolism and tumor-promoting pathways in squamous cancer. Li LY, Yang Q, Jiang YY, Yang W, Jiang Y, Li X, Hazawa M, Zhou B, Huang GW, Xu XE, Gery S, Zhang Y, Ding LW, Ho AS, Zumsteg ZS, Wang MR, Fullwood MJ, Freedland SJ, Meltzer SJ, Xu LY, Li EM, Koeffler HP, Lin DC. Nat Commun. 2021 Jul 16;12(1):4362. doi: 10.1038/s41467-021-24656-x. PubMed |
| Chromatin Remodeling of Colorectal Cancer Liver Metastasis is Mediated by an HGF-PU.1-DPP4 Axis. Wang L, Wang E, Prado Balcazar J, Wu Z, Xiang K, Wang Y, Huang Q, Negrete M, Chen KY, Li W, Fu Y, Dohlman A, Mines R, Zhang L, Kobayashi Y, Chen T, Shi G, Shen JP, Kopetz S, Tata PR, Moreno V, Gersbach C, Crawford G, Hsu D, Huang E, Bu P, Shen X. Adv Sci (Weinh). 2021 Oct;8(19):e2004673. doi: 10.1002/advs.202004673. Epub 2021 Aug 10. PubMed |
| Keap1 mutation renders lung adenocarcinomas dependent on Slc33a1. Romero R, Sanchez-Rivera FJ, Westcott PMK, Mercer KL, Bhutkar A, Muir A, Gonzalez Robles TJ, Lamboy Rodriguez S, Liao LZ, Ng SR, Li L, Colon CI, Naranjo S, Beytagh MC, Lewis CA, Hsu PP, Bronson RT, Vander Heiden MG, Jacks T. Nat Cancer. 2020 Jun;1(6):589-602. doi: 10.1038/s43018-020-0071-1. Epub 2020 Jun 8. PubMed |
| Runx3 is required for oncogenic Myc upregulation in p53-deficient osteosarcoma. Otani S, Date Y, Ueno T, Ito T, Kajikawa S, Omori K, Taniuchi I, Umeda M, Komori T, Toguchida J, Ito K. Oncogene. 2022 Jan;41(5):683-691. doi: 10.1038/s41388-021-02120-w. Epub 2021 Nov 22. PubMed |
| Super Enhancer-Mediated Upregulation of HJURP Promotes Growth and Survival of t(4;14)-Positive Multiple Myeloma. Jia Y, Zhou J, Tan TK, Chung TH, Chen Y, Chooi JY, Sanda T, Fullwood MJ, Xiong S, Toh SHM, Balan K, Wong RWJ, Lim JSL, Zhang E, Cai Z, Shen P, Chng WJ. Cancer Res. 2022 Feb 1;82(3):406-418. doi: 10.1158/0008-5472.CAN-21-0921. Epub 2021 Dec 10. PubMed |
| Deciphering the Design Rules of Toehold-Gated sgRNA for Conditional Activation of Gene Expression and Protein Degradation in Mammalian Cells. Hunt VM, Chen W. ACS Synth Biol. 2022 Jan 21;11(1):397-405. doi: 10.1021/acssynbio.1c00479. Epub 2022 Jan 7. PubMed |
| CRISPRi links COVID-19 GWAS loci to LZTFL1 and RAVER1. Fink-Baldauf IM, Stuart WD, Brewington JJ, Guo M, Maeda Y. EBioMedicine. 2022 Jan;75:103806. doi: 10.1016/j.ebiom.2021.103806. Epub 2022 Jan 6. PubMed |
| Human reproduction is regulated by retrotransposons derived from ancient Hominidae-specific viral infections. Xiang X, Tao Y, DiRusso J, Hsu FM, Zhang J, Xue Z, Pontis J, Trono D, Liu W, Clark AT. Nat Commun. 2022 Jan 24;13(1):463. doi: 10.1038/s41467-022-28105-1. PubMed |
| Methyltransferase METTL8 is required for 3-methylcytosine modification in human mitochondrial tRNAs. Lentini JM, Bargabos R, Chen C, Fu D. J Biol Chem. 2022 Apr;298(4):101788. doi: 10.1016/j.jbc.2022.101788. Epub 2022 Mar 3. PubMed |
| Targeting BPTF Sensitizes Pancreatic Ductal Adenocarcinoma to Chemotherapy by Repressing ABC-Transporters and Impairing Multidrug Resistance (MDR). Munoz Velasco R, Jimenez Sanchez P, Garcia Garcia A, Blanco Martinez-Illescas R, Pastor Senovilla A, Lozano Yague M, Trento A, Garcia-Martin RM, Navarro D, Sainz B Jr, Rodriguez Peralto JL, Sanchez-Arevalo Lobo VJ. Cancers (Basel). 2022 Mar 16;14(6). pii: cancers14061518. doi: 10.3390/cancers14061518. PubMed |
OVOL2 impairs RHO GTPase signaling to restrain mitosis and aggressiveness of Anaplastic Thyroid Cancer.
Gugnoni M, Manzotti G, Vitale E, Sauta E, Torricelli F, Reggiani F, Pistoni M, Piana S, Ciarrocchi A.
J Exp Clin Cancer Res. 2022 Mar 25;41(1):108. doi: 10.1186/s13046-022-02316-2.
PubMed
Associated Plasmids |
| The nuclear receptor ERR cooperates with the cardiogenic factor GATA4 to orchestrate cardiomyocyte maturation. Sakamoto T, Batmanov K, Wan S, Guo Y, Lai L, Vega RB, Kelly DP. Nat Commun. 2022 Apr 13;13(1):1991. doi: 10.1038/s41467-022-29733-3. PubMed |
| CRISPRi-mediated transcriptional silencing in iPSCs for the study of human brain development. Johansson PA, Adami A, Jakobsson J. STAR Protoc. 2022 Apr 13;3(2):101285. doi: 10.1016/j.xpro.2022.101285. eCollection 2022 Jun 17. PubMed |
| N6-methyladenosine regulated FGFR4 attenuates ferroptotic cell death in recalcitrant HER2-positive breast cancer. Zou Y, Zheng S, Xie X, Ye F, Hu X, Tian Z, Yan SM, Yang L, Kong Y, Tang Y, Tian W, Xie J, Deng X, Zeng Y, Chen ZS, Tang H, Xie X. Nat Commun. 2022 May 13;13(1):2672. doi: 10.1038/s41467-022-30217-7. PubMed |
| The risk variant rs11836367 contributes to breast cancer onset and metastasis by attenuating Wnt signaling via regulating NTN4 expression. Yang H, Ting X, Geng YH, Xie Y, Nierenberg JL, Huo YF, Zhou YT, Huang Y, Yu YQ, Yu XY, Li XF, Ziv E, Zhang H, Fang WG, Shen Y, Tian XX. Sci Adv. 2022 Jun 10;8(23):eabn3509. doi: 10.1126/sciadv.abn3509. Epub 2022 Jun 10. PubMed |
| Repression of HIV-1 reactivation mediated by CRISPR/dCas9-KRAB in lymphoid and myeloid cell models. da Costa LC, Bomfim LM, Dittz UVT, Velozo CA, da Cunha RD, Tanuri A. Retrovirology. 2022 Jun 22;19(1):12. doi: 10.1186/s12977-022-00600-9. PubMed |
| Exploring high-resolution chromatin interaction changes and functional enhancers of myogenic marker genes during myogenic differentiation. Long K, Li X, Su D, Zeng S, Li H, Zhang Y, Zhang B, Yang W, Li P, Li X, Wang X, Tang Q, Lu L, Jin L, Ma J, Li M. J Biol Chem. 2022 Aug;298(8):102149. doi: 10.1016/j.jbc.2022.102149. Epub 2022 Jul 2. PubMed |
| Transcriptomics, regulatory syntax, and enhancer identification in mesoderm-induced ESCs at single-cell resolution. Khateb M, Perovanovic J, Ko KD, Jiang K, Feng X, Acevedo-Luna N, Chal J, Ciuffoli V, Genzor P, Simone J, Haase AD, Pourquie O, Dell'Orso S, Sartorelli V. Cell Rep. 2022 Aug 16;40(7):111219. doi: 10.1016/j.celrep.2022.111219. PubMed |
| Transposon-activated POU5F1B promotes colorectal cancer growth and metastasis. Simo-Riudalbas L, Offner S, Planet E, Duc J, Abrami L, Dind S, Coudray A, Coto-Llerena M, Ercan C, Piscuoglio S, Andersen CL, Bramsen JB, Trono D. Nat Commun. 2022 Aug 20;13(1):4913. doi: 10.1038/s41467-022-32649-7. PubMed |
| Multimodal single cell sequencing implicates chromatin accessibility and genetic background in diabetic kidney disease progression. Wilson PC, Muto Y, Wu H, Karihaloo A, Waikar SS, Humphreys BD. Nat Commun. 2022 Sep 6;13(1):5253. doi: 10.1038/s41467-022-32972-z. PubMed |
| Nuclear receptor ligand screening in an iPSC-derived in vitro blood-brain barrier model identifies new contributors to leptin transport. Shi Y, Kim H, Hamann CA, Rhea EM, Brunger JM, Lippmann ES. Fluids Barriers CNS. 2022 Sep 21;19(1):77. doi: 10.1186/s12987-022-00375-3. PubMed |
| A novel oncogenic enhancer of estrogen receptor-positive breast cancer. Bao C, Duan J, Xie Y, Liu Y, Li P, Li J, Zhao H, Guo H, Men Y, Ren Y, Xu J, Wang G, Lu W. Mol Ther Nucleic Acids. 2022 Aug 24;29:836-851. doi: 10.1016/j.omtn.2022.08.029. eCollection 2022 Sep 13. PubMed |
Quantification of Genome Editing and Transcriptional Control Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in Human Cells.
Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB.
ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 10.1021/acssynbio.2c00156. Epub 2022 Sep 26.
PubMed
Associated Plasmids |
| KCNQ1OT1 promotes genome-wide transposon repression by guiding RNA-DNA triplexes and HP1 binding. Zhang X, Jiang Q, Li J, Zhang S, Cao Y, Xia X, Cai D, Tan J, Chen J, Han JJ. Nat Cell Biol. 2022 Nov;24(11):1617-1629. doi: 10.1038/s41556-022-01008-5. Epub 2022 Oct 20. PubMed |
| Defining cellular complexity in human autosomal dominant polycystic kidney disease by multimodal single cell analysis. Muto Y, Dixon EE, Yoshimura Y, Wu H, Omachi K, Ledru N, Wilson PC, King AJ, Eric Olson N, Gunawan MG, Kuo JJ, Cox JH, Miner JH, Seliger SL, Woodward OM, Welling PA, Watnick TJ, Humphreys BD. Nat Commun. 2022 Oct 30;13(1):6497. doi: 10.1038/s41467-022-34255-z. PubMed |
| CRISPRi screening reveals regulators of tau pathology shared between exosomal and vesicle-free tau. Polanco JC, Akimov Y, Fernandes A, Briner A, Hand GR, van Roijen M, Balistreri G, Gotz J. Life Sci Alliance. 2022 Oct 31;6(1):e202201689. doi: 10.26508/lsa.202201689. Print 2023 Jan. PubMed |
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