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Addgene

Reb_207 aka PAS630
(Plasmid #71235)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71235 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pETM11
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Reb locus
  • Alt name
    R body
  • Alt name
    Refractile bodies
  • Insert Size (bp)
    3709
  • Mutation
    RebA(S88P)
  • GenBank ID
    NC_005915.1
  • Tag / Fusion Protein
    • mNeonGreen to second copy RebB, soluble mCherry also downstream

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Reb_207 aka PAS630 was a gift from Pamela Silver (Addgene plasmid # 71235 ; http://n2t.net/addgene:71235 ; RRID:Addgene_71235)
  • For your References section:

    A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. Polka JK, Silver PA. ACS Synth Biol. 2016 Feb 3. 10.1021/acssynbio.5b00237 PubMed 26814170