Reb_207 aka PAS630
(Plasmid
#71235)
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PurposeReb_206 with RebA(S88P)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 71235 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepETM11
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameReb locus
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Alt nameR body
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Alt nameRefractile bodies
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Insert Size (bp)3709
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MutationRebA(S88P)
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GenBank IDNC_005915.1
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Tag
/ Fusion Protein
- mNeonGreen to second copy RebB, soluble mCherry also downstream
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Reb_207 aka PAS630 was a gift from Pamela Silver (Addgene plasmid # 71235 ; http://n2t.net/addgene:71235 ; RRID:Addgene_71235) -
For your References section:
A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. Polka JK, Silver PA. ACS Synth Biol. 2016 Feb 3. 10.1021/acssynbio.5b00237 PubMed 26814170