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Addgene

Reb_206 aka PAS629
(Plasmid #71234)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71234 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETM11
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Induce with 1mM IPTG at 37 for >4hrs
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Reb locus
  • Alt name
    R body
  • Alt name
    Refractile bodies
  • Insert Size (bp)
    3709
  • GenBank ID
    NC_005915.1
  • Promoter T7
  • Tag / Fusion Protein
    • mNeonGreen to second copy RebB, soluble mCherry also downstream

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Reb_206 aka PAS629 was a gift from Pamela Silver (Addgene plasmid # 71234 ; http://n2t.net/addgene:71234 ; RRID:Addgene_71234)

  • For your References section:

    A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. Polka JK, Silver PA. ACS Synth Biol. 2016 Feb 3. 10.1021/acssynbio.5b00237 PubMed 26814170
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