Reb_83 aka PAS624
(Plasmid #71229)

• Purpose: Reb_1 with RebA(G53A, D89G)
• Depositing Lab: Pamela Silver
• Publication: Polka et al ACS Synth Biol. 2016 Feb 3.

Backbone

• Vector backbone: pETM11
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Reb locus
• Alt name: R body
• Alt name: Refractile bodies
• Insert Size (bp): 1750
• Mutation: RebA(G53A, D89G)
• GenBank ID: NC_005915.1
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: NheI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Resource Information

• Supplemental Documents:
  • Reb1 Map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note-This plasmid was originally reported to have a C-terminal frameshift mutation in RebB (Polka et al ACS Synth Biol. 2016).. Addgene's quality control sequences indicate that the frameshift mutation is not present and the plasmid contains the entire RebB gene.