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Addgene

Reb_81 aka PAS622
(Plasmid #71227)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71227 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETM11
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Reb locus
  • Alt name
    R body
  • Alt name
    Refractile bodies
  • Insert Size (bp)
    1750
  • Mutation
    RebB(T28A, D72G), RebA(G53A)
  • GenBank ID
    NC_005915.1
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequences and the listed Genbank sequence [NC_005915.1]. The depositor noted that these discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Reb_81 aka PAS622 was a gift from Pamela Silver (Addgene plasmid # 71227 ; http://n2t.net/addgene:71227 ; RRID:Addgene_71227)

  • For your References section:

    A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. Polka JK, Silver PA. ACS Synth Biol. 2016 Feb 3. 10.1021/acssynbio.5b00237 PubMed 26814170
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