Reb_80 aka PAS621
(Plasmid #71226)

• Purpose: Reb_1 with RebB(I67T, N84I)
• Depositing Lab: Pamela Silver
• Publication: Polka et al ACS Synth Biol. 2016 Feb 3.

Backbone

• Vector backbone: pETM11
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Reb locus
• Alt name: R body
• Alt name: Refractile bodies
• Insert Size (bp): 1750
• Mutation: RebB(I67T, N84I)
• GenBank ID: NC_005915.1
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: NheI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Resource Information

• Supplemental Documents:
  • Reb1 Map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequences and the listed Genbank sequence [NC_005915.1]. The depositor noted that these discrepancies do NOT affect plasmid function.