Reb_1 aka PAS619
(Plasmid #71224)

• Purpose: Expresses wild type R bodies from C. taeniospiralis in pETM11 backbone
• Depositing Lab: Pamela Silver
• Publication: Polka et al ACS Synth Biol. 2016 Feb 3.

Backbone

• Vector backbone: pETM11
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Reb locus
• Alt name: R body
• Alt name: Refractile bodies
• Insert Size (bp): 1750
• Mutation: RebA G53A
• GenBank ID: NC_005915.1
• Promoter: T7
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • Reb1 Map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.