Skip to main content
Addgene

pLenti-EF1a-SPdCas9-EGFP-2A-Blast
(Plasmid #71215)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71215 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    3rd generation lentiviral vector
  • Total vector size (bp) 14631
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dSpCas9-EGFP
  • Species
    Synthetic
  • Tag / Fusion Protein
    • dSpCas9-EGFP (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Contains a Valine insertion upstream of EGFP and a LYK deletion at the C-terminus of EGFP. These changes do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-EF1a-SPdCas9-EGFP-2A-Blast was a gift from Yonglun Luo (Addgene plasmid # 71215 ; http://n2t.net/addgene:71215 ; RRID:Addgene_71215)

  • For your References section:

    Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases. Lin L, Liu Y, Xu F, Huang J, Daugaard TF, Petersen TS, Hansen B, Ye L, Zhou Q, Fang F, Yang L, Li S, Floe L, Jensen KT, Shrock E, Chen F, Yang H, Wang J, Liu X, Xu X, Bolund L, Nielsen AL, Luo Y. Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011. 10.1093/gigascience/giy011 PubMed 29635374
Related items:
Commonly requested with: