pSGE_709_S2-1-Pal_dCP
(Plasmid
#71162)
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PurposeLuciferase reporter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71162 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneunknown
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Vector typeInsect Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name709_S2-1-Pal_dCP
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SpeciesSynthetic
- Promoter DSCP Drosophila synthetic core promoter
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSGE_709_S2-1-Pal_dCP was a gift from Alexander Stark (Addgene plasmid # 71162 ; http://n2t.net/addgene:71162 ; RRID:Addgene_71162) -
For your References section:
Transcriptional regulators form diverse groups with context-dependent regulatory functions. Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A. Nature. 2015 Nov 9. doi: 10.1038/nature15545. 10.1038/nature15545 PubMed 26550828
