pJK240
(Plasmid #71127)

• Purpose: Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli
• Depositing Lab: T. J. Kappock
• Publication: Francois et al Biochemistry. 2006 Nov 14;45(45):13487-99.

Backbone

• Vector backbone: pTWIN1
• Backbone manufacturer: NEB
• Backbone size w/o insert (bp): 7375
• Total vector size (bp): 7966
• Modifications to backbone: His6 tag added to chitin-binding domain (CBD) of Mxe Intein
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: citrate synthase
• Alt name: aarA
• Alt name: gltA
• Species: Acetobacter aceti 1023
• GenBank ID: ABF85671 KDE21011
• Promoter: T7
• Tags / Fusion Proteins:
  • GyrA intein (C terminal on backbone)
  • His6 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: Mxe intein reverse primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry