pJK136
(Plasmid #71122)

• Purpose: Expression of Acetobacter aceti 1023 citrate synthase (AarA) in E. coli
• Depositing Lab: T. J. Kappock
• Publication: Francois et al Biochemistry. 2006 Nov 14;45(45):13487-99.

Backbone

• Vector backbone: pET23a
• Backbone size w/o insert (bp): 3666
• Total vector size (bp): 4935
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: citrate synthase
• Alt name: AarA
• Alt name: CS
• Alt name: GltA
• Species: Acetobacter aceti 1023
• GenBank ID: KDE21011 ABF85671
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

DNA sequencing revealed three single-base differences from the earlier published sequence (GenBank accession number M34830), leading to two changes in the deduced amino acid sequence: codon 85 is GCC (Ala), not GAC (Asp), and codon 106 is GCG (Ala), not GTG (Val). There is also a silent difference in codon 50 (Phe)