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Addgene

pJK136
(Plasmid #71122)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71122 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET23a
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 4935
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    citrate synthase
  • Alt name
    AarA
  • Alt name
    CS
  • Alt name
    GltA
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE21011 ABF85671
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

DNA sequencing revealed three single-base differences from the earlier published sequence (GenBank accession number M34830), leading to two changes in the deduced amino acid sequence: codon 85 is GCC (Ala), not GAC (Asp), and codon 106 is GCG (Ala), not GTG (Val). There is also a silent difference in codon 50 (Phe)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK136 was a gift from T. J. Kappock (Addgene plasmid # 71122 ; http://n2t.net/addgene:71122 ; RRID:Addgene_71122)

  • For your References section:

    Structure of a NADH-insensitive hexameric citrate synthase that resists acid inactivation. Francois JA, Starks CM, Sivanuntakorn S, Jiang H, Ransome AE, Nam JW, Constantine CZ, Kappock TJ. Biochemistry. 2006 Nov 14;45(45):13487-99. 10.1021/bi061083k PubMed 17087502
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