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Addgene

pJK287
(Plasmid #71120)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71120 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6657
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    formyl-CoA:oxalate CoA-transferase
  • Alt name
    UctB
  • Alt name
    CoAT2
  • Alt name
    frc
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    ABG35150 109629858
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK287 was a gift from T. J. Kappock (Addgene plasmid # 71120 ; http://n2t.net/addgene:71120 ; RRID:Addgene_71120)

  • For your References section:

    Formyl-coenzyme A (CoA):oxalate CoA-transferase from the acidophile Acetobacter aceti has a distinctive electrostatic surface and inherent acid stability. Mullins EA, Starks CM, Francois JA, Sael L, Kihara D, Kappock TJ. Protein Sci. 2012 May;21(5):686-96. doi: 10.1002/pro.2054. Epub 2012 Mar 29. 10.1002/pro.2054 PubMed 22374910
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