pDEST-FRT/T0-GFP-BRCA1
(Plasmid #71116)

• Purpose: mammalian expression of GFP-BRCA1 siRNA resistant construct
• Depositing Lab: Daniel Durocher
• Publication: Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature16142.

Backbone

• Vector backbone: pDEST-GFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BRCA1
• Species: H. sapiens (human)
• Entrez Gene: BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
• Promoter: CMV/TO
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • Annotated GenBank file
• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

siRNA resistant sequence: CAGCTACCCTagtATaATc
The BRCA1 insert contains the following mutations (residue numbering relative to the plasmid's open reading frame): P1565L, E1732G, A1836T, K1877R, and S2307G