pDEST-FRT-TO-GFP-PALB2 KR
(Plasmid #71107)

• Purpose: mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)
• Depositing Lab: Daniel Durocher
• Publication: Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature16142.

Backbone

• Vector backbone: pDEST-GFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PALB2
• Species: H. sapiens (human)
• Mutation: first 8 Lysine residues mutated to Arginine (K14-16-18-20-25-30-43-45)
• Entrez Gene: PALB2 (a.k.a. BROVCA5, FANCN, PNCA3)
• Promoter: CMV/TO
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • Annotated SnapGene file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA