pFOSGE_COF2E12
(Plasmid
#70940)
-
PurposeGateway entry clone
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 70940 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepDONR221
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4762
-
Vector typeGateway entry vector
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSu(var)3-3
-
Alt nameFBgn0260397
-
Alt nameCG17149, FBtr0074825
-
SpeciesD. melanogaster (fly)
-
Insert Size (bp)2670
-
Entrez GeneSu(var)3-3 (a.k.a. CG17149, dLsd1, Dmel/HDM, Dmel/LSD1, Dmel\CG17149, Dmel\LSD1, Hdm, Lsd1, SU(VAR)3-3, Su-var(3)3, Suvar(3)3)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFOSGE_COF2E12 was a gift from Alexander Stark (Addgene plasmid # 70940 ; http://n2t.net/addgene:70940 ; RRID:Addgene_70940) -
For your References section:
Transcriptional regulators form diverse groups with context-dependent regulatory functions. Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A. Nature. 2015 Nov 9. doi: 10.1038/nature15545. 10.1038/nature15545 PubMed 26550828