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PurposeExpresses phosphomimetic mouse ATP-citrate lyase in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70768 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEF6
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5800
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameATP Citrate Lyase
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Alt nameAcly
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SpeciesM. musculus (mouse)
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Insert Size (bp)3300
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MutationS455D
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Entrez GeneAcly (a.k.a. A730098H14Rik)
- Promoter EF1alpha
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Tag
/ Fusion Protein
- myc-epitope (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF6-Acly(S455D) was a gift from Kathryn Wellen (Addgene plasmid # 70768 ; http://n2t.net/addgene:70768 ; RRID:Addgene_70768) -
For your References section:
Akt-dependent metabolic reprogramming regulates tumor cell histone acetylation. Lee JV, Carrer A, Shah S, Snyder NW, Wei S, Venneti S, Worth AJ, Yuan ZF, Lim HW, Liu S, Jackson E, Aiello NM, Haas NB, Rebbeck TR, Judkins A, Won KJ, Chodosh LA, Garcia BA, Stanger BZ, Feldman MD, Blair IA, Wellen KE. Cell Metab. 2014 Aug 5;20(2):306-19. doi: 10.1016/j.cmet.2014.06.004. Epub 2014 Jul 3. 10.1016/j.cmet.2014.06.004 PubMed 24998913