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Addgene

pEF6-Acly
(Plasmid #70765)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70765 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF6
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATP Citrate Lyase
  • Alt name
    Acly
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3300
  • Entrez Gene
    Acly (a.k.a. A730098H14Rik)
  • Promoter EF1alpha
  • Tag / Fusion Protein
    • myc-epitope (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF6-Acly was a gift from Kathryn Wellen (Addgene plasmid # 70765 ; http://n2t.net/addgene:70765 ; RRID:Addgene_70765)

  • For your References section:

    Akt-dependent metabolic reprogramming regulates tumor cell histone acetylation. Lee JV, Carrer A, Shah S, Snyder NW, Wei S, Venneti S, Worth AJ, Yuan ZF, Lim HW, Liu S, Jackson E, Aiello NM, Haas NB, Rebbeck TR, Judkins A, Won KJ, Chodosh LA, Garcia BA, Stanger BZ, Feldman MD, Blair IA, Wellen KE. Cell Metab. 2014 Aug 5;20(2):306-19. doi: 10.1016/j.cmet.2014.06.004. Epub 2014 Jul 3. 10.1016/j.cmet.2014.06.004 PubMed 24998913
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