pET28-hXRCC1
(Plasmid #70759)

• Purpose: bacterial expression of human XRCC1
• Depositing Lab: Primo Schaer
• Publication: Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1520.

Backbone

• Vector backbone: pET28c(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 7141
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: XRCC1
• Species: H. sapiens (human)
• Insert Size (bp): 1914
• GenBank ID: NM_006297.2
• Entrez Gene: XRCC1 (a.k.a. RCC, SCAR26)
• Promoter: T7/lac operon
• Tag / Fusion Protein:
  • 6-HIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (destroyed during cloning)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: pBRrevBam
• 3′ sequencing primer: T7 terminal

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry