pET28HIS-hAPE1
(Plasmid #70757)

• Purpose: bacterial expression of human APE1
• Depositing Lab: Primo Schaer
• Publication: Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1520.

Backbone

• Vector backbone: pET28c(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5367
• Total vector size (bp): 6259
• Modifications to backbone: modified MCS including C-terminal thrombin cleavage site and 6xHIS tag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: APEX1
• Alt name: APE1
• Alt name: HAP1
• Alt name: REF-1
• Species: H. sapiens (human)
• Insert Size (bp): 960
• GenBank ID: NM_080649.2
• Entrez Gene: APEX1 (a.k.a. APE, APE1, APEN, APEX, APX, HAP1, REF1)
• Promoter: T7/lac operon
• Tag / Fusion Protein:
  • 6-HIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBRrevBam
• 3′ sequencing primer: T7 terminal

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry