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Addgene

pGEX-KG-mTmod3-150-352_domain
(Plasmid #70755)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70755 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-KG
  • Backbone size w/o insert (bp) 5006
  • Total vector size (bp) 5606
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    re-transform to BL21 for IPTG-induced protein production
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tropomodulin3 150-352 AA fragment
  • Alt name
    U-Tmod
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    612
  • Entrez Gene
    Tmod3 (a.k.a. U-Tmod, UTMOD)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer ggg ctg gca agc cac gtt tgg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-KG-mTmod3-150-352_domain was a gift from Weiping Han (Addgene plasmid # 70755 ; http://n2t.net/addgene:70755 ; RRID:Addgene_70755)

  • For your References section:

    Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Lim CY, Bi X, Wu D, Kim JB, Gunning PW, Hong W, Han W. Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951. 10.1038/ncomms6951 PubMed 25575350
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