pCI-BCD7-lacI-ssrA P62U18C50T16 v40-4a (496)
(Plasmid
#70737)
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Purposeplasmid for building 5n1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70737 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonen/a
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)KL740
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Growth instructionsgrow at 30 in KL740 lambda-repressor strain to avoid mutations
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namelacI-ssrA
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-BCD7-lacI-ssrA P62U18C50T16 v40-4a (496) was a gift from Richard Murray (Addgene plasmid # 70737 ; http://n2t.net/addgene:70737 ; RRID:Addgene_70737) -
For your References section:
Rapid cell-free forward engineering of novel genetic ring oscillators. Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, Maerkl SJ. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771. 10.7554/eLife.09771 PubMed 26430766