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Addgene

pLacO1-BCD7-tetR-ssrA P42U18C45T13 v40-5a (497)
(Plasmid #70733)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70733 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    n/a

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    MG1655Z1
  • Growth instructions
    grow in lac repressor strain at low temperature to avoid mutations
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tetR-ssrA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLacO1-BCD7-tetR-ssrA P42U18C45T13 v40-5a (497) was a gift from Richard Murray (Addgene plasmid # 70733 ; http://n2t.net/addgene:70733 ; RRID:Addgene_70733)

  • For your References section:

    Rapid cell-free forward engineering of novel genetic ring oscillators. Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, Maerkl SJ. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771. 10.7554/eLife.09771 PubMed 26430766
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