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Addgene

5n1 (560)
(Plasmid #70730)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70730 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    n/a

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    minimize passages; can accumulate deletion mutations
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    srpR-ssrA

Gene/Insert 2

  • Gene/Insert name
    lambdaCI-ssrA

Gene/Insert 3

  • Gene/Insert name
    phlF-ssrA

Gene/Insert 4

  • Gene/Insert name
    lacI-ssrA

Gene/Insert 5

  • Gene/Insert name
    tetR-ssrA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5n1 (560) was a gift from Richard Murray (Addgene plasmid # 70730 ; http://n2t.net/addgene:70730 ; RRID:Addgene_70730)
  • For your References section:

    Rapid cell-free forward engineering of novel genetic ring oscillators. Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, Maerkl SJ. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771. 10.7554/eLife.09771 PubMed 26430766