PC4_3dad1h
(Plasmid #70699)

• Purpose: Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on dad1
• Depositing Lab: Stephen Harrison
• Publication: Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-43. Epub 2005 Jan 10.

Backbone

• Vector backbone: pST39
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For transformation and plasmid preparation add 0.1 % glucose to the LB medium
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DASH / Dam1 complex
• Alt name: dad1, duo1, spc34, dam1, hsk3, dad4, dad3, dad2, spc19, ask1
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: DAM1 (a.k.a. YGR113W)
• Entrez Gene: ASK1 (a.k.a. YKL052C)
• Entrez Gene: DUO1 (a.k.a. YGL061C)
• Entrez Gene: SPC34 (a.k.a. YKR037C)
• Entrez Gene: SPC19 (a.k.a. YDR201W)
• Entrez Gene: DAD1 (a.k.a. YDR016C)
• Entrez Gene: DAD2 (a.k.a. YKR083C, HSK1)
• Entrez Gene: DAD4 (a.k.a. YDR320C-A, HSK2)
• Entrez Gene: HSK3 (a.k.a. YKL138C-A)
• Entrez Gene: DAD3 (a.k.a. YBR233W-A)
• Promoter: T7
• Tag / Fusion Protein:
  • his-tag on C-terminus of dad1

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Miranda JJ

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry