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Addgene

pRL1075
(Plasmid #70688)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70688 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    see comments
  • Selectable markers
    Erythromycin in cyanobacteria

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MCS: EcoRV-BglII-XhoI-BssHII-SpeI-BsiWI-AvrII-AflII-BstBI and potentially other sites

sacB was derived from pUM24 (Ried, J. L., and A. Colimer. Gene 57:239-246, 1987) and from pUCD800 (Gay, et al. 1985 J. Bacteriol. 164:918-921). The polylinker came, in large part, from the plC20R polylinker (Marsh et al.. 1984: Gene 32: 481-485) and a polylinker region of plasmid pJRD184 (Heusterspreute et al.. 1985: Gene 37: 299-304).

The erythromycin-resistance gene came from Horinouchi, S., and Weisblum, B. (J. Bacteriol. 150: 804-814, 1982).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRL1075 was a gift from Peter Wolk (Addgene plasmid # 70688 ; http://n2t.net/addgene:70688 ; RRID:Addgene_70688)

  • For your References section:

    Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Black TA, Cai Y, Wolk CP. Mol Microbiol. 1993 Jul;9(1):77-84. PubMed 8412673
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