pRL1058
(Plasmid #70686)

• Purpose: Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.
• Depositing Lab: Peter Wolk
• Publication: Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5355-9.

Backbone

• Vector backbone: Transposon Tn5, variously modified
• Modifications to backbone: 5395 bp of the 6489 bp are a transposon whose ends are linked by an origin of transfer.
• Selectable markers: Bleomycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin and Streptomycin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Transposon Tn5-1058

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Tn5 derivative Tn5-1058 (in plasmid pRL1058) contains a p15A oriV, and its antibiotic-resistance determinants are driven by an Amaranthus hybridus ribulose bisphosphate carboxylase promoter (see pRL439 is this series of plasmids), providing large numbers of transposon mutants. The XbaI site near the L end of Tn5 is the sole unmethylated XbaI in the construct, allowing the placement of luciferases (e.g., Vibrio fischeri luxAB as in pRL1063a). An oriT for conjugal transfer is positioned between the L and R ends of the Tn.