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pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)
(Plasmid #70669)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70669 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E2F1 lacking residues 284 to 359
  • Alt name
    RBP3; E2F-1; RBAP1; RBBP3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1137
  • Mutation
    lacks amin acids 284 to 359
  • GenBank ID
    NM_005225
  • Entrez Gene
    E2F1 (a.k.a. E2F-1, RBAP1, RBBP3, RBP3)
  • Promoter cmv
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII BamHI (not destroyed)
  • 3′ cloning site BamHI EcoRI (not destroyed)
  • 5′ sequencing primer 5’-TAA TAC GAC TCA CTA TAG GG-3’
  • 3′ sequencing primer 5’-TAGAAGGCACAGTCGAGG-3’
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    I did sub clone the E2F1 mutant from the E2F1 gene provided to me by Dr. Joseph R. Nevins

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI) was a gift from Maria Alvarado-Kristensson (Addgene plasmid # 70669 ; http://n2t.net/addgene:70669 ; RRID:Addgene_70669)

  • For your References section:

    Nuclear localization of gamma-tubulin affects E2F transcriptional activity and S-phase progression. Hoog G, Zarrizi R, von Stedingk K, Jonsson K, Alvarado-Kristensson M. FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 2011 Jul 25. 10.1096/fj.11-187484 PubMed 21788450
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