pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)
(Plasmid #70668)

• Purpose: Human mutant of E2F1 lacking amino acids 2 to 126
• Depositing Lab: Maria Alvarado-Kristensson
• Publication: Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 2011 Jul 25.

Backbone

• Vector backbone: pcDNA3.1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: E2F1 lacking residues 2 to 126
• Alt name: RBP3; E2F-1; RBAP1; RBBP3
• Species: H. sapiens (human)
• Insert Size (bp): 993
• Mutation: lacks amino acids 2-126
• GenBank ID: NM_005225
• Entrez Gene: E2F1 (a.k.a. E2F-1, RBAP1, RBBP3, RBP3)
• Promoter: cmv
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII BamHI (not destroyed)
• 3′ cloning site: BamHI EcoRI (not destroyed)
• 5′ sequencing primer: 5’-TAA TAC GAC TCA CTA TAG GG-3’
• 3′ sequencing primer: 5’-TAGAAGGCACAGTCGAGG-3’

Resource Information

• A portion of this plasmid was derived from a plasmid made by: I did sub clone the E2F1 mutant from the E2F1 gene provided to me by Dr. Joseph R. Nevins

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry