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pBS/U6/RNAi gamma-tubulin shRNA mouse
(Plasmid #70666)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70666 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript/U6
  • Vector type
    Mammalian Expression ; U6 promoter

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´
  • Alt name
    CDCBM4, GCP-1, TUBG, TUBGCP1; AI504772, Tubgl
  • gRNA/shRNA sequence
    TUBG1 and TUBG2
  • Species
    M. musculus (mouse)
  • GenBank ID
    NM_134024.2 NM_134028.2
  • Entrez Gene
    Tubg1 (a.k.a. 1500010O08Rik, GCP-1, Tubg)
  • Entrez Gene
    Tubg2 (a.k.a. AI504772, Tubg, Tubgl)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ApaI (destroyed during cloning)
  • 3′ cloning site ApaI (destroyed during cloning)
  • 5′ sequencing primer 5’-TTACATGATAGGCTTGGAT-3’
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note there is a single nucleotide mismatch between the depositor's sequence and Addgene's quality control sequence. This mismatch does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS/U6/RNAi gamma-tubulin shRNA mouse was a gift from Maria Alvarado-Kristensson (Addgene plasmid # 70666 ; http://n2t.net/addgene:70666 ; RRID:Addgene_70666)

  • For your References section:

    Nuclear localization of gamma-tubulin affects E2F transcriptional activity and S-phase progression. Hoog G, Zarrizi R, von Stedingk K, Jonsson K, Alvarado-Kristensson M. FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 2011 Jul 25. 10.1096/fj.11-187484 PubMed 21788450
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