pMXs-DDX3X
(Plasmid #70648)

• Purpose: Expresses WT DDX3X in mammalian cells under blasticidin selection.
• Depositing Lab: David Sabatini
• Publication: Wang et al Science. 2015 Oct 15. pii: aac7041.

Backbone

• Vector backbone: pMXs
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DDX3X
• Species: H. sapiens (human)
• Entrez Gene: DDX3X (a.k.a. CAP-Rf, DBX, DDX14, DDX3, HLP2, MRX102, MRXSSB)
• Tag / Fusion Protein:
  • 3x FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: RTVfor

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry