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Addgene

R777-E139 Hs.NF1
(Plasmid #70423)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70423 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDonor-255
  • Backbone size w/o insert (bp) 2693
  • Vector type
    Gateway Entry Clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NF1
  • Alt name
    NM_000267.3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8457
  • Entrez Gene
    NF1 (a.k.a. NFNS, VRNF, WSS)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13F 23-mer (5'-CCCAGTCACGACGTTGTAAAACG)
  • 3′ sequencing primer attLR 23-mer (5'-TAACATCAGAGATTTTGAGACAC)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

sequence codon optimized for cloning stability

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R777-E139 Hs.NF1 was a gift from Dominic Esposito (Addgene plasmid # 70423 ; http://n2t.net/addgene:70423 ; RRID:Addgene_70423)
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