R777-E129 Hs.MDM2
(Plasmid
#70413)
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PurposeGateway ORF clone of human MDM2 [NM_002392.5] with stop codon (for native or N-terminal fusions)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70413 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDonor-255
- Backbone size w/o insert (bp) 2693
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Vector typeGateway Entry Clone
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMDM2
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Alt nameNM_002392.5
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SpeciesH. sapiens (human)
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Insert Size (bp)1494
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Entrez GeneMDM2 (a.k.a. ACTFS, HDMX, LSKB, hdm2)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F 23-mer (5'-CCCAGTCACGACGTTGTAAAACG)
- 3′ sequencing primer attLR 23-mer (5'-TAACATCAGAGATTTTGAGACAC) (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
g1146a silent mutation not noted in Refseq
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
R777-E129 Hs.MDM2 was a gift from Dominic Esposito (Addgene plasmid # 70413 ; http://n2t.net/addgene:70413 ; RRID:Addgene_70413)