R777-E123 Hs.MAP2K2
(Plasmid
#70407)
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PurposeGateway ORF clone of human MAP2K2 [NM_030662.3] with stop codon (for native or N-terminal fusions)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70407 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDonor-255
- Backbone size w/o insert (bp) 2693
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Vector typeGateway Entry Clone
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMAP2K2
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Alt nameNM_030662.3
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SpeciesH. sapiens (human)
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Insert Size (bp)1203
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Entrez GeneMAP2K2 (a.k.a. CFC4, MAPKK2, MEK2, MKK2, PRKMK2)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F 23-mer (5'-CCCAGTCACGACGTTGTAAAACG)
- 3′ sequencing primer attLR 23-mer (5'-TAACATCAGAGATTTTGAGACAC) (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
c453t and c660a silent mutations not noted in Refseq
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
R777-E123 Hs.MAP2K2 was a gift from Dominic Esposito (Addgene plasmid # 70407 ; http://n2t.net/addgene:70407 ; RRID:Addgene_70407)