R777-E112 Hs.IRS2-nostop
(Plasmid
#70396)
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PurposeGateway ORF clone of codon optimized human IRS2 [NM_003749] without stop codon (for C-terminal fusions)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70396 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDonor-255
- Backbone size w/o insert (bp) 2693
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Vector typeGateway Entry Clone
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIRS2
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Alt nameNM_003749
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SpeciesH. sapiens (human)
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Insert Size (bp)4014
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Entrez GeneIRS2 (a.k.a. IRS-2)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F 23-mer (5'-CCCAGTCACGACGTTGTAAAACG)
- 3′ sequencing primer attLR 23-mer (5'-TAACATCAGAGATTTTGAGACAC) (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
sequence codon optimized for cloning stability
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
R777-E112 Hs.IRS2-nostop was a gift from Dominic Esposito (Addgene plasmid # 70396 ; http://n2t.net/addgene:70396 ; RRID:Addgene_70396)