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Addgene

R777-E091 Hs.FLT3
(Plasmid #70375)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70375 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDonor-255
  • Backbone size w/o insert (bp) 2693
  • Vector type
    Gateway Entry Clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLT3
  • Alt name
    NM_004119.2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2982
  • Mutation
    T227M (human SNP)
  • Entrez Gene
    FLT3 (a.k.a. CD135, FLK-2, FLK2, STK1)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13F 23-mer (5'-CCCAGTCACGACGTTGTAAAACG)
  • 3′ sequencing primer attLR 23-mer (5'-TAACATCAGAGATTTTGAGACAC)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

c288t silent mutation not noted in Refseq; c680t (T227M) SNP found in alternate human genome sequences

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R777-E091 Hs.FLT3 was a gift from Dominic Esposito (Addgene plasmid # 70375 ; http://n2t.net/addgene:70375 ; RRID:Addgene_70375)
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