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Addgene

pRL648
(Plasmid #70283)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70283 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC
  • Backbone size (bp) 2686
  • Vector type
    Bacterial shuttle vector
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alphaMCR
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    From pAH484 (see pRL439, Addgene plasmid 70258) and from transposon Tn5, connected with a 6-bp segment from pUC8, and all set between a pair of BamHI and SmaI sites in symmetric polylinker L.HEHl from Elhai and Wolk, 1988, PMID 2851487.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional references:
Elhai J, Wolk CP (1988) A versatile class of positive selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68: 119-138
Kuritz T, Ernst A, Black TA, Wolk CP (1993) High-resolution mapping of genetic loci of Anabaena PCC 7120 required for photosynthesis and nitrogen fixation. Mol Microbiol 8: 101-110

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRL648 was a gift from Peter Wolk (Addgene plasmid # 70283 ; http://n2t.net/addgene:70283 ; RRID:Addgene_70283)

  • For your References section:

    Analysis of a Het- mutation in Anabaena sp. strain PCC 7120 implicates a secondary metabolite in the regulation of heterocyst spacing. Black TA, Wolk CP. J Bacteriol. 1994 Apr;176(8):2282-92. PubMed 8157596
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