pRL439
(Plasmid
#70258)
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PurposeSource of psbA promoter of Amaranthus hybridus, used as a strong promoter of cyanobacterial genes
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70258 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC8
- Total vector size (bp) 2736
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepsbA promoter
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SpeciesAmaranthus hybridus
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRL439 was a gift from Peter Wolk (Addgene plasmid # 70258 ; http://n2t.net/addgene:70258 ; RRID:Addgene_70258) -
For your References section:
A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Elhai J, Wolk CP. Gene. 1988 Aug 15;68(1):119-38. 0378-1119(88)90605-1 [pii] PubMed 2851487