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Addgene

pJK392
(Plasmid #70236)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70236 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET23a
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 4137
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    phosphoribosylaminoimidazole carboxylase
  • Alt name
    PurE
  • Alt name
    TDE0687
  • Alt name
    aminoimidazole ribonucleotide carboxylase
  • Species
    Treponema denticola
  • Mutation
    His40Asn
  • GenBank ID
    NP_971299.1

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Used to produce the catalytically inactive H40N mutant.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK392 was a gift from T. J. Kappock (Addgene plasmid # 70236 ; http://n2t.net/addgene:70236 ; RRID:Addgene_70236)

  • For your References section:

    Treponema denticola PurE Is a bacterial AIR carboxylase. Tranchimand S, Starks CM, Mathews II, Hockings SC, Kappock TJ. Biochemistry. 2011 May 31;50(21):4623-37. doi: 10.1021/bi102033a. Epub 2011 May 6. 10.1021/bi102033a PubMed 21548610
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