pJK376
(Plasmid #70235)

• Purpose: Protein production from a synthetic gene encoding Treponema denticola ATCC 35405 PurE (UniProt Q73PV9_TREDE)
• Depositing Lab: T. J. Kappock
• Publication: Tranchimand et al Biochemistry. 2011 May 31;50(21):4623-37. doi: 10.1021/bi102033a. Epub 2011 May 6.

Backbone

• Vector backbone: pET23a
• Backbone size w/o insert (bp): 3666
• Total vector size (bp): 4137
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: phosphoribosylaminoimidazole carboxylase
• Alt name: PurE
• Alt name: TDE0687
• Alt name: aminoimidazole ribonucleotide carboxylase
• Species: Treponema denticola
• GenBank ID: NP_971299.1
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This protein is the same as referenced in NCBI WP_002681904.1, which is incorrectly annotated as a N5-carboxyaminoimidazole ribonucleotide mutase. Functional expression studies show that it does not possess this activity and it instead has the characteristic reactivity of a class II PurE or 5-aminoimidazole ribonucleotide (AIR) carboxylase.