pGZ110 (pFA6a-3FLAG-MNase-TRP1)
(Plasmid
#70233)
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PurposeChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70233 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a-3HA-Trp1
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Modifications to backboneThe 3HA coding sequencing was dropped out of backbone with AscI and PacI and replaced with 3FLAG-MNase.
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Vector typeYeast genomic targeting
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberUnknown
Gene/Insert
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Gene/Insert name3xFLAG tag and aa 83-231 of Mnase
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Alt nameChEC-tagging vector
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Alt name3FLAG-MNase
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Insert Size (bp)525
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGZ110 (pFA6a-3FLAG-MNase-TRP1) was a gift from Steven Henikoff (Addgene plasmid # 70233 ; http://n2t.net/addgene:70233 ; RRID:Addgene_70233) -
For your References section:
ChEC-seq kinetics discriminates transcription factor binding sites by DNA sequence and shape in vivo. Zentner GE, Kasinathan S, Xin B, Rohs R, Henikoff S. Nat Commun. 2015 Oct 22;6:8733. doi: 10.1038/ncomms9733. 10.1038/ncomms9733 PubMed 26490019
Map uploaded by the depositor.
