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pGZ108 (pFA6a-3FLAG-MNase-kanMX6)
(Plasmid #70231)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70231 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a-3HA-kanMX6
  • Modifications to backbone
    The 3HA coding sequencing was dropped out of backbone with AscI and PacI and replaced with 3FLAG-MNase.
  • Vector type
    Yeast genomic targeting
  • Selectable markers
    Neomycin (select with G418) ; KanR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    3xFLAG tag and aa 83-231 of Mnase
  • Alt name
    ChEC-tagging vector
  • Alt name
    3FLAG-MNase
  • Insert Size (bp)
    525

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGZ108 (pFA6a-3FLAG-MNase-kanMX6) was a gift from Steven Henikoff (Addgene plasmid # 70231 ; http://n2t.net/addgene:70231 ; RRID:Addgene_70231)

  • For your References section:

    ChEC-seq kinetics discriminates transcription factor binding sites by DNA sequence and shape in vivo. Zentner GE, Kasinathan S, Xin B, Rohs R, Henikoff S. Nat Commun. 2015 Oct 22;6:8733. doi: 10.1038/ncomms9733. 10.1038/ncomms9733 PubMed 26490019
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