3XFLAG-NSarQ27G-VMAn-FKBP
(Plasmid #70229)

• Purpose: Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing
• Depositing Lab: Perry Howard
• Publication: Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 2014 Jan 30.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6490
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: alpha Sarcin (N-terminal portion)
• Species: Synthetic
• Insert Size (bp): 81
• Mutation: Changed Gln 27 to Gly
• GenBank ID: X53394.1
• Promoter: CMV
• Tags / Fusion Proteins:
  • VMAn (C terminal on insert)
  • Linker Peptide (C terminal on insert)
  • FK506- and rapamycin-binding protein (FKBP) (C terminal on insert)
  • 3XFLAG (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: N-Sar-For
• 3′ sequencing primer: N-Sar-Rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry