pcDNA3-FRB-VMAc-CGFP
(Plasmid #70226)

• Purpose: Expresses C-terminal portion of GFP along with VMA intein for protein splicing
• Depositing Lab: Perry Howard
• Publication: Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 2014 Jan 30.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6253
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: eGFP
• Species: Synthetic
• Insert Size (bp): 333
• Mutation: Changed Ile 129 to Cys, Deleted amino acids 1-128
• GenBank ID: LC008492.1
• Promoter: CMV
• Tags / Fusion Proteins:
  • FKBP-rapamycin binding (FRB) domain of the mammalian target of rapamycin (mTOR) kinase (N terminal on insert)
  • Linker Peptide (N terminal on insert)
  • VMAc (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: C-Sar For
• 3′ sequencing primer: C-Sar Rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry