pcDNA3-FRB-VMAc-CSarcin
(Plasmid
#70223)
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PurposeExpresses VMA intein with C-terminal portion of alpha-sarcin
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70223 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 6300
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namealpha Sarcin (C-terminal portion)
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SpeciesSynthetic
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Insert Size (bp)369
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MutationChanged Asp 28 to Cys, deleted amino acids 1-27
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GenBank IDX53394.1
- Promoter CMV
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Tags
/ Fusion Proteins
- FKBP-rapamycin binding (FRB) domain of the mammalian target of rapamycin (mTOR) kinase (N terminal on insert)
- Linker Peptide (N terminal on insert)
- VMAc (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer C-Sar For
- 3′ sequencing primer C-Sar Rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-FRB-VMAc-CSarcin was a gift from Perry Howard (Addgene plasmid # 70223 ; http://n2t.net/addgene:70223 ; RRID:Addgene_70223) -
For your References section:
Conditional protein splicing of alpha-sarcin in live cells. Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL. Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 2014 Jan 30. 10.1039/c3mb70387h PubMed 24481070