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Addgene

pcDNA3-FRB-VMAc-CSarcin
(Plasmid #70223)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70223 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    alpha Sarcin (C-terminal portion)
  • Species
    Synthetic
  • Insert Size (bp)
    369
  • Mutation
    Changed Asp 28 to Cys, deleted amino acids 1-27
  • GenBank ID
    X53394.1
  • Promoter CMV
  • Tags / Fusion Proteins
    • FKBP-rapamycin binding (FRB) domain of the mammalian target of rapamycin (mTOR) kinase (N terminal on insert)
    • Linker Peptide (N terminal on insert)
    • VMAc (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-FRB-VMAc-CSarcin was a gift from Perry Howard (Addgene plasmid # 70223 ; http://n2t.net/addgene:70223 ; RRID:Addgene_70223)
  • For your References section:

    Conditional protein splicing of alpha-sarcin in live cells. Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL. Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 2014 Jan 30. 10.1039/c3mb70387h PubMed 24481070