Skip to main content
Addgene

pACUH-GFP11x7-mCherry-α-tubulin
(Plasmid #70218)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70218 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pACUH
  • Backbone manufacturer
    Yuh-Nung Jan lab
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP11x7-mCherry-α-tubulin
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2538
  • Tags / Fusion Proteins
    • GFP11x7 (N terminal on insert)
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TCAACTGCAACTACTGA
  • 3′ sequencing primer GCTTTAAATCTCTGTAGGTAGTTTGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACUH-GFP11x7-mCherry-α-tubulin was a gift from Bo Huang (Addgene plasmid # 70218 ; http://n2t.net/addgene:70218 ; RRID:Addgene_70218)

  • For your References section:

    Versatile protein tagging in cells with split fluorescent protein. Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. 10.1038/ncomms11046 PubMed 26988139
Related items:
Commonly requested with: