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Addgene

P3U2C5T2-v1-2 (Pl-lacO1-tetR) (136p)
(Plasmid #70212)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70212 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBEST

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MG1655Z1
  • Growth instructions
    Must grow in lacIQ-type strain; can add 0.1% glucose to tighten repression
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tetR

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    P3U2C5T2-v1-2 (Pl-lacO1-tetR) (136p) was a gift from Richard Murray (Addgene plasmid # 70212 ; http://n2t.net/addgene:70212 ; RRID:Addgene_70212)

  • For your References section:

    Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4. 10.1021/sb400131a PubMed 24303785
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